World Central Kitchen added that its convoy was hit “despite coordinating movements with the IDF.”
A Palestinian and a dual U.S.-Canadian citizen were also killed in the strike, the group said.
The IDF said it was conducting “an in-depth examination at the highest levels” into the “tragic incident” but did not say whether it was responsible, according to the Times of Israel.
One of the aid workers killed has been identified by authorities as 43-year-old Australian Lalzawmi Frankcom.
Australian Prime Minister Anthony Albanese described Frankcom’s death as “completely unacceptable” and said he had summoned the Israeli ambassador.
“We want full accountability for this because this is a tragedy that should never have occurred,” Albanese said.